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preadipocyte basal medium  (PromoCell)


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    PromoCell preadipocyte basal medium
    Preadipocyte Basal Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preadipocyte basal medium/product/PromoCell
    Average 93 stars, based on 5 article reviews
    preadipocyte basal medium - by Bioz Stars, 2026-03
    93/100 stars

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    (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

    Journal: Frontiers in Endocrinology

    Article Title: Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes

    doi: 10.3389/fendo.2024.1396965

    Figure Lengend Snippet: (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

    Article Snippet: The isolated inguinal white adipose tissue stromal vascular fraction was resuspended in Preadipocyte Basal Medium (BulletKit, Lonza, PT-8002) with the addition of L-glutamine, GA-1000 and FBS (SingleQuots, part of BulletKit, Lonza, PT-8002) and seeded in 12–24-wells (Thermo Scientific, 150628 and 142475) depending on the experiment.

    Techniques: Isolation, Light Microscopy, Immunofluorescence, Staining, Marker, Expressing, Standard Deviation, MANN-WHITNEY